PLoS Biology November 25, 2025

Benchmarking with synthetic communities provides a baseline for virus-host inferences from Hi-C proximity linking

by Rokaiya Nurani Shatadru, Natalie E. Solonenko, Christine L. Sun, Matthew B. Sullivan Microbiomes influence diverse ecosystems, and viruses increasingly appear to impose key constraints. While viromics has expanded genomic catalogs, host identification for these viruses remains challenging due to the limitations in scaling cultivation-based approaches and the uncertain reliability and relative low resolution of in silico predictions – particularly for understudied viral taxa. Towards this, Hi